Journal: International journal of molecular sciences

Article Title: Effect of Flavonoids on MCP-1 Expression in Human Coronary Artery Endothelial Cells and Impact on MCP-1-Dependent Migration of Human Monocytes.

doi: 10.3390/ijms242216047

Figure Lengend Snippet: Figure 7. Effect of recombinant MCP-1 (A) and a neutralizing antibody (nab) directed against MCP-1 (B) on monocyte migration. Migration of THP-1 cells was determined with Boyden chamber assay toward increasing MCP-1 concentrations (A) or toward CM from HCAEC (B). In (A), medium containing recombinant MCP-1 added to the lower chamber was used to induce monocyte migration. In (B), CM from HCAEC previously treated with IL-1β or vehicle for 24 h was incubated with MCP-1 antibody (1 µg/mL) or the appropriate isotype control IgG2B (1 µg/mL) for 1 h after removal from the cells. Thereafter, migration of THP-1 monocytes toward CM was initiated. In both settings (A,B), migration was run for 6 h. Monocyte migration toward vehicle-containing medium (A) or toward CM of IL-1β-treated HCAEC containing IgG2B (B) were set to 100%. Data are presented as means ± SEM of n = 5–6 (A) or n = 6 (B) of three independent experiments each. ** p ≤0.01, *** p ≤0.001 vs. vehicle control; one-way ANOVA with Dunnett’s post hoc test (A). *** p ≤0.001 vs. vehicle control (leftmost white bar); ### p ≤0.001 vs. IL-1β-stimulated cells; one-way ANOVA with Bonferroni’s post hoc test (B).

Article Snippet: IgG2B Isotype Control (#MAB004; RRID:AB_357346) and CCL2/JE/MCP-1 Antibody (#MAB679; RRID:AB_2071559) were obtained from R&D Systems (Wiesbaden, Germany).

Techniques: Recombinant, Migration, Boyden Chamber Assay, Incubation, Control

Journal: Cell Reports

Article Title: Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity

doi: 10.1016/j.celrep.2018.08.006

Figure Lengend Snippet:

Article Snippet: The human/mouse Anti-UCP1 antibody (MAB6158, monoclonal Mouse IgG 2B Clone # 536435, R&D Systems) was conjugated to Alexa647 (ThermoFisher Alexa Fluor 647 antibody labeling kit A20186) and incubated with adipocytes at 1:300 for 1 h. Mouse IgG2B Alexa Fluor 647-conjugated Isotype Control antibody (R&D systems) was used as a control.

Techniques: Control, Recombinant, Blocking Assay, Antibody Labeling, Software, Flow Cytometry

Journal: Nature Communications

Article Title: Single-cell transcriptome and translatome dual-omics reveals potential mechanisms of human oocyte maturation

doi: 10.1038/s41467-022-32791-2

Figure Lengend Snippet: a Time-lapse image showing the progressive maturation of human GV oocytes in hRec-OOSP2 containing medium (top row) and control medium (bottom row) with indicated time (h). Red arrows indicate the occurrence of GVBD or PBE. b Pie chart showing the maturation rate of hRec-OOSP2 treated oocytes and control oocytes. n = 24 (21 pairs of independent donors, each pair of oocytes was from the same donor. Chi-square = 2.9484, p = 0.08586, Chi-square test). c Time-lapse images showing the progressive maturation of human GV oocytes in antibody against OOSP2 (ab-OOSP2) containing medium (top row) and control medium (ig-Control, bottom row) with indicated time (h). Red arrows indicate the occurrence of GVBD or PBE. d Pie chart shows the maturation rate of ab-OOSP2 treated oocytes and igG control oocytes. n = 16 (14 pairs of independent donors, each pair of oocytes was from the same donor. Chi-square = 4.57143, p = 0.03251, Chi-square test). e PBE duration of each pair of oocytes in hRec-OOSP2 and control oocytes. PBE duration over 72 h is counted as not matured. f PBE duration of each pair of oocytes in ab-OOSP2 and the control oocytes. PBE duration over 72 h is counted as not matured.

Article Snippet: OOSP2 antibody (17343-1-AP, Proteintech) and control immunoglobulins (IgG) (FNSA-0106, Finetest) were purified in advance.

Techniques: